Twenty mg free CO2 per litre is considered the utmost permissible focus for trout (larger concentrations may cause kidney issues) and 25 mg free CO2 per litre is the utmost for carp (if the acid capability is 0.5 mmol per litre at a pH of up to 4.5). The sensitivity of fish to free carbon dioxide declines with rising acid capacity of water. In water of low O2 and high CO2, where gaseous alternate at the respiratory surface is proscribed, the fish enhance their ventilation rate, turn into restless, lose equilibrium, and should die. Ground waters from limestone or chalk strata often comprise a number of tens of mg of free CO2 per litre, and this can be necessary the place nicely water is used for fish culture.In stagnant floor waters the CO2
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