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roots of 100 and sixty maxillary molars had been instrumented and divided into completely different teams, according to the obturation method utilized -lateral condensation, Microseal system, Touch’n heat + Ultrafil system, and Tagger’s hybrid approach- and the extension of the remaining obturation materials . Ten additional roots had been employed as management. The tradition medium BHI was inoculated with the microorganism Enterococcus faecalis and placed in touch with the coronal portion of roots; the same sterile culture medium was positioned in

 

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